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Figure 611 UV spectra of saturated solutions of miconazole as a function of pH: (a) sample; (b) reference [Avdeef, A, Curr Topics Med Chem, 1, 277 351 (2001) Reproduced with permission from Bentham Science Publishers, Ltd]

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Figure 611 shows the measured absorption spectra of miconazole (reference and sample) As precipitation takes place to varying degrees at different pH values, the spectra of the sample solutions change in optical densities, according to Beer s law This can be clearly seen in Fig 612 for the sample spectra, where the sample spectra have the lowest OD values at pH 90 and systematically show higher OD values

Figure 612 Correction of the apparent solubility pH pro le (solid curves) for the effect of DMSO and/or aggregation: (a) chlorpromazine; (b) terfenadine; (c) piroxicam; (d) phenazopyridine [Avdeef, A, Curr Topics Med Chem, 1, 277 351 (2001) Reproduced with permission from Bentham Science Publishers, Ltd]

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as pH is lowered, a pattern consistent with that of an weak base The changing OD values indicate that solubility changes with pH

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65 CORRECTION FOR THE DMSO EFFECT BY THE D-SHIFT METHOD 651 DMSO Binding to the Uncharged Form of a Compound

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